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correlative cryo workflow solution  (Carl Zeiss)


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    Structured Review

    Carl Zeiss correlative cryo workflow solution
    Schematic overview of the <t>cryo-/LP-CLEM</t> <t>workflow.</t> Graphene liquid cell preparation is completely automated. The machine etches away the copper (7°C), transfers the graphene to a TEM grid via loop-assisted transfer and seals the GLCs by blotting away excess liquid. The reaction starts once the liquid pockets are sealed. Live fluorescence microscopy can be used to determine not only the location, but the specific time to image at high resolution, at which point the process is arrested by rapid vitrification. ROIs are located using cryo-fluorescence microscopy and hereafter they are imaged at nanometer resolution (cryo-TEM). The process is reinitiated inside the microscope by heating-up the grid using a cryo-holder, and the reaction dynamics are directly recorded (LP-TEM). To confirm that GLCs remain intact and retain liquid after TEM observation, fluorescence microscopy is used.
    Correlative Cryo Workflow Solution, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/correlative+cryo+workflow+solution/bio_rxiv__2023__05__08__539575-150-16-15?v=Carl+Zeiss
    Average 90 stars, based on 1 article reviews
    correlative cryo workflow solution - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "A Cryo-/Liquid Phase Correlative Light Electron Microscopy Workflow to Visualize Crystallization Processes in Graphene Liquid Cells"

    Article Title: A Cryo-/Liquid Phase Correlative Light Electron Microscopy Workflow to Visualize Crystallization Processes in Graphene Liquid Cells

    Journal: bioRxiv

    doi: 10.1101/2023.05.08.539575

    Schematic overview of the cryo-/LP-CLEM workflow. Graphene liquid cell preparation is completely automated. The machine etches away the copper (7°C), transfers the graphene to a TEM grid via loop-assisted transfer and seals the GLCs by blotting away excess liquid. The reaction starts once the liquid pockets are sealed. Live fluorescence microscopy can be used to determine not only the location, but the specific time to image at high resolution, at which point the process is arrested by rapid vitrification. ROIs are located using cryo-fluorescence microscopy and hereafter they are imaged at nanometer resolution (cryo-TEM). The process is reinitiated inside the microscope by heating-up the grid using a cryo-holder, and the reaction dynamics are directly recorded (LP-TEM). To confirm that GLCs remain intact and retain liquid after TEM observation, fluorescence microscopy is used.
    Figure Legend Snippet: Schematic overview of the cryo-/LP-CLEM workflow. Graphene liquid cell preparation is completely automated. The machine etches away the copper (7°C), transfers the graphene to a TEM grid via loop-assisted transfer and seals the GLCs by blotting away excess liquid. The reaction starts once the liquid pockets are sealed. Live fluorescence microscopy can be used to determine not only the location, but the specific time to image at high resolution, at which point the process is arrested by rapid vitrification. ROIs are located using cryo-fluorescence microscopy and hereafter they are imaged at nanometer resolution (cryo-TEM). The process is reinitiated inside the microscope by heating-up the grid using a cryo-holder, and the reaction dynamics are directly recorded (LP-TEM). To confirm that GLCs remain intact and retain liquid after TEM observation, fluorescence microscopy is used.

    Techniques Used: Fluorescence, Microscopy

    Cryo-/LP-CLEM workflow to visualize crystallization processes inside a GLC. a) TEM overview imaged two days after thawing, overlaid with live-FM (green) to indicate the GLCs. Insert shows a high magnification LP-TEM image of the GLC in the blue box. b) SAED pattern taken at the position indicated by the black dashed circle in . Inner ring in the DP shows the contribution of the [100] plane on NaCl and outer ring the contribution of the graphene. c) Enlargement of the area marked by the orange box in were multiple GLCs are present (green, yellow and purple boxes) and a crystal that is not encapsulated by graphene (red box). D-f) Large crystal inside a GLC imaged at multiple time points after thawing (3d: 2 days; 3e: 5 days; 3f: 7 days) shows morphological changes. Graphene wrinkles (close yellow arrow), outline of the GLC (dotted yellow line) and the intensity gradient of the liquid surrounding the crystal (open yellow arrow) are visible at all time points. g) Two different but interconnected GLCs imaged two days after thawing (open and closed purple arrows). h) Condensation (solid arrow), and phase transformation (open purple arrow) observed within the pockets five days after thawing. I) Cubic NaCl crystal resulting from an Ostwald ripening process observed seven days after thawing j) Liquid pockets (green dashed circles) containing an amorphous-like phase. k) Nucleation within the amorphous phase contained in the GLCs (green striped circles). l) Dissolution of small crystals (top circle) and further crystallization (bottom circle) inside the GLCs. m) Crystal not encapsulated by graphene outlined by the red dotted line shows only minimum morphological changes five (3n) and seven (3o) days after thawing. Accumulative dose: a) 0.75e - /Å ; c, d, g, j, m) 0.15 e - /Å ; e, h, k, n) 0.30 e - /Å ; f, i, l, o) 0.45 e - /Å
    Figure Legend Snippet: Cryo-/LP-CLEM workflow to visualize crystallization processes inside a GLC. a) TEM overview imaged two days after thawing, overlaid with live-FM (green) to indicate the GLCs. Insert shows a high magnification LP-TEM image of the GLC in the blue box. b) SAED pattern taken at the position indicated by the black dashed circle in . Inner ring in the DP shows the contribution of the [100] plane on NaCl and outer ring the contribution of the graphene. c) Enlargement of the area marked by the orange box in were multiple GLCs are present (green, yellow and purple boxes) and a crystal that is not encapsulated by graphene (red box). D-f) Large crystal inside a GLC imaged at multiple time points after thawing (3d: 2 days; 3e: 5 days; 3f: 7 days) shows morphological changes. Graphene wrinkles (close yellow arrow), outline of the GLC (dotted yellow line) and the intensity gradient of the liquid surrounding the crystal (open yellow arrow) are visible at all time points. g) Two different but interconnected GLCs imaged two days after thawing (open and closed purple arrows). h) Condensation (solid arrow), and phase transformation (open purple arrow) observed within the pockets five days after thawing. I) Cubic NaCl crystal resulting from an Ostwald ripening process observed seven days after thawing j) Liquid pockets (green dashed circles) containing an amorphous-like phase. k) Nucleation within the amorphous phase contained in the GLCs (green striped circles). l) Dissolution of small crystals (top circle) and further crystallization (bottom circle) inside the GLCs. m) Crystal not encapsulated by graphene outlined by the red dotted line shows only minimum morphological changes five (3n) and seven (3o) days after thawing. Accumulative dose: a) 0.75e - /Å ; c, d, g, j, m) 0.15 e - /Å ; e, h, k, n) 0.30 e - /Å ; f, i, l, o) 0.45 e - /Å

    Techniques Used: Crystallization Assay, Transformation Assay



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    correlative cryo workflow solution  (Carl Zeiss)
    90
    Carl Zeiss correlative cryo workflow solution
    Schematic overview of the <t>cryo-/LP-CLEM</t> <t>workflow.</t> Graphene liquid cell preparation is completely automated. The machine etches away the copper (7°C), transfers the graphene to a TEM grid via loop-assisted transfer and seals the GLCs by blotting away excess liquid. The reaction starts once the liquid pockets are sealed. Live fluorescence microscopy can be used to determine not only the location, but the specific time to image at high resolution, at which point the process is arrested by rapid vitrification. ROIs are located using cryo-fluorescence microscopy and hereafter they are imaged at nanometer resolution (cryo-TEM). The process is reinitiated inside the microscope by heating-up the grid using a cryo-holder, and the reaction dynamics are directly recorded (LP-TEM). To confirm that GLCs remain intact and retain liquid after TEM observation, fluorescence microscopy is used.
    Correlative Cryo Workflow Solution, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/correlative+cryo+workflow+solution/bio_rxiv__2023__05__08__539575-150-16-15?v=Carl+Zeiss
    Average 90 stars, based on 1 article reviews
    correlative cryo workflow solution - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic overview of the cryo-/LP-CLEM workflow. Graphene liquid cell preparation is completely automated. The machine etches away the copper (7°C), transfers the graphene to a TEM grid via loop-assisted transfer and seals the GLCs by blotting away excess liquid. The reaction starts once the liquid pockets are sealed. Live fluorescence microscopy can be used to determine not only the location, but the specific time to image at high resolution, at which point the process is arrested by rapid vitrification. ROIs are located using cryo-fluorescence microscopy and hereafter they are imaged at nanometer resolution (cryo-TEM). The process is reinitiated inside the microscope by heating-up the grid using a cryo-holder, and the reaction dynamics are directly recorded (LP-TEM). To confirm that GLCs remain intact and retain liquid after TEM observation, fluorescence microscopy is used.

    Journal: bioRxiv

    Article Title: A Cryo-/Liquid Phase Correlative Light Electron Microscopy Workflow to Visualize Crystallization Processes in Graphene Liquid Cells

    doi: 10.1101/2023.05.08.539575

    Figure Lengend Snippet: Schematic overview of the cryo-/LP-CLEM workflow. Graphene liquid cell preparation is completely automated. The machine etches away the copper (7°C), transfers the graphene to a TEM grid via loop-assisted transfer and seals the GLCs by blotting away excess liquid. The reaction starts once the liquid pockets are sealed. Live fluorescence microscopy can be used to determine not only the location, but the specific time to image at high resolution, at which point the process is arrested by rapid vitrification. ROIs are located using cryo-fluorescence microscopy and hereafter they are imaged at nanometer resolution (cryo-TEM). The process is reinitiated inside the microscope by heating-up the grid using a cryo-holder, and the reaction dynamics are directly recorded (LP-TEM). To confirm that GLCs remain intact and retain liquid after TEM observation, fluorescence microscopy is used.

    Article Snippet: After vitrification, the TEM grids were loaded into a universal TEM cryo-holder (349559-8100-010) using the ZEISS Correlative Cryo Workflow solution, which fit into the PrepDek® (PP3010Z, Quorum technologies, Laughton, UK).

    Techniques: Fluorescence, Microscopy

    Cryo-/LP-CLEM workflow to visualize crystallization processes inside a GLC. a) TEM overview imaged two days after thawing, overlaid with live-FM (green) to indicate the GLCs. Insert shows a high magnification LP-TEM image of the GLC in the blue box. b) SAED pattern taken at the position indicated by the black dashed circle in . Inner ring in the DP shows the contribution of the [100] plane on NaCl and outer ring the contribution of the graphene. c) Enlargement of the area marked by the orange box in were multiple GLCs are present (green, yellow and purple boxes) and a crystal that is not encapsulated by graphene (red box). D-f) Large crystal inside a GLC imaged at multiple time points after thawing (3d: 2 days; 3e: 5 days; 3f: 7 days) shows morphological changes. Graphene wrinkles (close yellow arrow), outline of the GLC (dotted yellow line) and the intensity gradient of the liquid surrounding the crystal (open yellow arrow) are visible at all time points. g) Two different but interconnected GLCs imaged two days after thawing (open and closed purple arrows). h) Condensation (solid arrow), and phase transformation (open purple arrow) observed within the pockets five days after thawing. I) Cubic NaCl crystal resulting from an Ostwald ripening process observed seven days after thawing j) Liquid pockets (green dashed circles) containing an amorphous-like phase. k) Nucleation within the amorphous phase contained in the GLCs (green striped circles). l) Dissolution of small crystals (top circle) and further crystallization (bottom circle) inside the GLCs. m) Crystal not encapsulated by graphene outlined by the red dotted line shows only minimum morphological changes five (3n) and seven (3o) days after thawing. Accumulative dose: a) 0.75e - /Å ; c, d, g, j, m) 0.15 e - /Å ; e, h, k, n) 0.30 e - /Å ; f, i, l, o) 0.45 e - /Å

    Journal: bioRxiv

    Article Title: A Cryo-/Liquid Phase Correlative Light Electron Microscopy Workflow to Visualize Crystallization Processes in Graphene Liquid Cells

    doi: 10.1101/2023.05.08.539575

    Figure Lengend Snippet: Cryo-/LP-CLEM workflow to visualize crystallization processes inside a GLC. a) TEM overview imaged two days after thawing, overlaid with live-FM (green) to indicate the GLCs. Insert shows a high magnification LP-TEM image of the GLC in the blue box. b) SAED pattern taken at the position indicated by the black dashed circle in . Inner ring in the DP shows the contribution of the [100] plane on NaCl and outer ring the contribution of the graphene. c) Enlargement of the area marked by the orange box in were multiple GLCs are present (green, yellow and purple boxes) and a crystal that is not encapsulated by graphene (red box). D-f) Large crystal inside a GLC imaged at multiple time points after thawing (3d: 2 days; 3e: 5 days; 3f: 7 days) shows morphological changes. Graphene wrinkles (close yellow arrow), outline of the GLC (dotted yellow line) and the intensity gradient of the liquid surrounding the crystal (open yellow arrow) are visible at all time points. g) Two different but interconnected GLCs imaged two days after thawing (open and closed purple arrows). h) Condensation (solid arrow), and phase transformation (open purple arrow) observed within the pockets five days after thawing. I) Cubic NaCl crystal resulting from an Ostwald ripening process observed seven days after thawing j) Liquid pockets (green dashed circles) containing an amorphous-like phase. k) Nucleation within the amorphous phase contained in the GLCs (green striped circles). l) Dissolution of small crystals (top circle) and further crystallization (bottom circle) inside the GLCs. m) Crystal not encapsulated by graphene outlined by the red dotted line shows only minimum morphological changes five (3n) and seven (3o) days after thawing. Accumulative dose: a) 0.75e - /Å ; c, d, g, j, m) 0.15 e - /Å ; e, h, k, n) 0.30 e - /Å ; f, i, l, o) 0.45 e - /Å

    Article Snippet: After vitrification, the TEM grids were loaded into a universal TEM cryo-holder (349559-8100-010) using the ZEISS Correlative Cryo Workflow solution, which fit into the PrepDek® (PP3010Z, Quorum technologies, Laughton, UK).

    Techniques: Crystallization Assay, Transformation Assay